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. 2018 Apr 3;8:5451. doi: 10.1038/s41598-018-23806-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heterologous expression of MG detoxifying enzymes in yeast mutants confers oxidative stress tolerance: BY4741 wild type yeast cells, GLYIKO, GLYIIKO and D-LDHKO mutant yeast cells containing empty vector pYES2 were grown in presence of oxidative stress (A). These mutant cells were transformed with their respective mutant genes cloned in pYES2 vector and the complemented yeast cells were grown in presence of oxidative stress and their growth was monitored along with wild type BY4741 cells (B). MG levels were estimated in all of these samples after 30 hours and contribution of these genes in detoxification of MG was compared (C).