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. 2018 Apr 3;8:5451. doi: 10.1038/s41598-018-23806-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional complementation of MG detoxification genes in respective yeast mutant confirms the contribution of different genes in MG detoxification: Yeast cells mutant of GLYI, GLYII and D-DLH were transformed with pYES2-AtGLYI, pYES2-AtGLYII and pYES2-AtD-LDH respectively and were grown in Ura⁻ SD solid media with different supplements to check for their contribution to MG detoxification. Wild type BY4741 cells containing empty vector pYES2 were used for comparison. (i) Pictorial representation of various strains used. (ii) Medium supplemented with 20% glucose. (iii) medium supplemented with 20% glucose and 1 mM MG. (iv) Medium supplemented with 20% galactose; (v) Medium supplemented with 20% galactose and 1 mM MG. (vi) Medium supplemented with 20% galactose and 2 mM MG. (vii) Medium supplemented with 20% galactose and 3 mM MG. (viii) Medium supplemented with 20% galactose and 4 mM MG. (ix) Medium supplemented with 20% galactose and 5 mM MG.