Table 1.
The calibration curves, linearity ranges, LODs, LOQs and recoveries of nine compounds (P = 95%, k = 2).
| Compounds | Regression equation | R2 | Linearity range(µg/ml) | MDL (µg/ml) | MQL (µg/ml) | Recovery | |
|---|---|---|---|---|---|---|---|
| Means ± U(%) | RSD(%) | ||||||
| Clitorin | y = 0.2674 × − 0.0154 | 0.9999 | 4–128 | 0.09 | 0.3 | 100.8 ± 0.5 | 3.12 |
| Rutin | y = 0.3479 × + 0.0736 | 0.9997 | 4–128 | 0.08 | 0.2 | 98.1 ± 0.9 | 3.91 |
| Isoquercitrin | y = 0.4362 × − 0.0876 | 0.9996 | 1.5–48 | 0.04 | 0.1 | 104.5 ± 0.1 | 1.32 |
| Quercetin-3-O-D-glucosyl]-(1-2)-L-rhamnoside | y = 0.3458 × − 0.3234 | 0.9994 | 5–160 | 0.07 | 0.2 | 98.9 ± 0.6 | 3.76 |
| Kaempferol-3-O-rutinoside | y = 0.4151 × − 0.0989 | 0.9994 | 2.5–120 | 0.12 | 0.4 | 99.8 ± 1.0 | 3.46 |
| Kaempferol-7-O-β-D-glucopyranoside | y = 0.7537 × − 0.8358 | 0.9998 | 2.5–80 | 0.04 | 0.1 | 100.7 ± 0.3 | 4.49 |
| Apigenin-7-O-Glucoside | y = 0.719 × − 0.3239 | 0.9997 | 2.5–80 | 0.07 | 0.2 | 100.2 ± 0.8 | 0.95 |
| Quercetin-3-O-[2-O-(6-O-p-hydroxyl-E-coumaroyl)-D-glucosyl]-(1-2)-L-rhamnoside | y = 0.3604 × − 0.2522 | 0.9999 | 6–192 | 0.09 | 0.3 | 96.9 ± 0.3 | 0.79 |
| 3-O-{2-O-[6-O-(p-hydroxyl-E-coumaroyl)-glucosyl]-(1-2)rhamnosyl kaempferol | y = 0.5507 × − 0.3791 | 0.9998 | 4–128 | 0.05 | 0.1 | 103.3 ± 0.5 | 1.04 |