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. 2018 Apr 3;8:5569. doi: 10.1038/s41598-018-23909-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regression analysis of ACP in wheat seed and plant dry weight at seedling stage. (A) Wheat seed produced under different nitrogen level. Four nitrogen level: N0 (0 kg/ha), N168 (168 kg/ha), N240 (240 kg/ha, the usual nitrogen fertilizer level for winter wheat production in the North China Plain) and N300 (300 kg/ha). (B) Wheat seed came from big spike of experiment A. (C and D) Wheat seed came from different parts of spike (top 1/4, middle 1/3 and bottom 1/4) under N0 and N240 treatments, respectively. (E) Wheat seed produced under different irrigation after anthesis. Two water level: normal irrigation and no irrigation with control rainfall. (F) Thirty wheat seed lots included 27 wheat cultivars seeds produced in normal regional trial, two wheat cultivars seeds produced in drought condition and one wheat cultivars seeds produced in low nitrogen condition. ACP: the absolute content of protein (mg seed⁻¹).