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. 2018 Apr 3;8:5576. doi: 10.1038/s41598-018-23528-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximal uptake rate (V_max) values and steady-state fluxes for different pre-growth conditions. (A) V_max values were measured in a 5 s zero-trans uptake assay with 200 mM glucose as substrate. Pre-growth was done on 2% of the carbon source indicated. Prior to the experiment cells were washed in medium without carbon source and incubated for 3′ at 30 °C without a carbon source. Experiments were done in triplicate and shown are the averages with standard deviation. (B) Glucose and ethanol concentrations were measured during two hours at 30 °C following a glucose pulse. Protein concentration remained constant throughout the experiment. From this data, specific exchange fluxes were calculated. Shown is the average and standard deviation of two incubations per pre-growth condition.