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. 2018 Mar 1;8(3):435–449.

Figure 5.

Figure 5

Tumor-suppressing roles of miR-532 on CRC cells are rescued by IGF-1R overexpression. SW480 and HCT116 cells were cotransfected with miR-532 mimics and pcDNA3.1-IGF-1R or pcDNA3.1. After incubation for different times, transfected cells were used in the subsequent assays. A. At 72 h post-transfection, the protein level of IGF-1R in the indicated cells was measured using western blot analysis. *P < 0.05 vs. miR-NC. #P < 0.05 vs. miR-532 mimics + pcDNA3.1-IGF-1R. B-F. The CCK-8 assay, colony formation assay, flow cytometric analysis and Transwell assay were applied to determine the proliferation, colony formation, apoptosis, migration, and invasion of the previously described cells. *P < 0.05 vs. miR-NC. #P < 0.05 vs. miR-532 mimics + pcDNA3.1-IGF-1R.