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. 2018 Mar 1;8(3):526–537.

Figure 2.

Figure 2

HAND2-AS1 deficiency reversed energy stress-regulated proliferation and apoptosis. (A) lncRNA HAND2-AS1 was downregulated by transfection of specific siRNAs in MG-63 and SAOS-2 cells. (B, C) MG-63 (A) and SAOS-2 (B) cells transfected with si-HAND2-AS1 or si-NC were cultured in glucose starvation or normal condition for different days as indicated, and then subjected to cell proliferation analysis. (D) Colony formation assay was performed after transfection of indicated siRNAs for 48 h in both osteosarcoma cells. (E) TUNEL assay was performed to detect the effect of HAND2-AS1 knockdown on cell apoptosis in MG-63 and SAOS-2 cells.