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. 2018 Mar 1;8(3):551–565.

Figure 5.

Figure 5

MMP13 knocked-down reversed the effects of GOLM1 in NSCLC. A. H1975 and HCC827 cells were transfected with GOLM1 or co-transfected with siMMP13 and GOLM1. The expression of MMP13 was determined by qRT-PCR analysis. B. Both H1975 and HCC8271 cells were transfected with GOLM1, or co-transfected with siMMP13 and GOLM1, and then were seeded into 96 well plates. After 24 h, 48 h, 72 h and 96 h, the MTT assay was performed to analysis cell proliferation. C. Colony formation assays of H1975 and HCC827 cells. Representative images for each treatment were shown. D. H1975 and HCC827 cells co-transfected with siMMP13 and GOLM1 were subjected to wound closure assay. The percentage of wound closure was quantified (right panel). Scale bar: 200 μm. E. Transwell invasion assay was performed after transfection of H1975 and HCC827 cells with siMMP13 and GOLM1. The invaded cells were stained with crystal violet and counted. Scale bar: 200 μm. *P < 0.01, **P < 0.01 as compared to control and ##P < 0.01 as compared to GOLM1.