Figure 9.
The effect of RP11-396F22.1 downregulation on Cpne8 expression. The effect of RP11-396F22.1 downregulation on Cpne8 expression was detected by qRT-PCR (A) and Western blotting (B). Interference group showed decreased expression of RP11-396F22.1 and increased levels of Cpne8 mRNA and protein expression. (C) The effect of RP11-396F22.1 downregulation on miRNA-32 expression was detected by qRT-PCR. Effects of overexpression and downregulation of miRNA-32 on Cpne8 expression were detected by qRT-PCR (D) and Western blotting (E). (F) Downregulation of Cpne8 restored the migration ability of Hela cells after RP11-396F22.1 knockdown. *P<0.05 vs NC and **P<0.01 vs NC.