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Rat NP cells were treated with PGE-2 and the expression of Hh molecules were analyzed with RT-PCR. The expression of Ihh was not changed after PGE-2 treatment. However, PGE-2 significantly suppressed Shh expression of (A). Western blotting analysis showed that PGE-2 suppressed the protein expression of Shh (B). IHC study of the IVD samples with an antibody to Shh showed a much stronger immunostaining in Cox-2-/- mice than in WT ones (C). *, P < 0.05. Error bars, S.D.
