Figure 4.

Effects of miR-223 on the apoptosis of ASMCs. A. The effects on ASMC apoptosis were determined using a cytofluorometric apoptosis kit and subsequent flow cytometry to distinguish PI- and Annexin V-positive cells. This showed that apoptosis was not affected by transfection with a miR-223 mimic or miR-223 inhibitor. B. The mean proportion of apoptotic cells in each treatment group (both early and late apoptosis cells) from three independent experiments. Data are means ± SD (**P < 0.01; * P < 0.05; n = 5).