Skip to main content
. 2018 Mar 15;10(3):744–752.

Figure 6.

Figure 6

The effects of targeting IGF-1R by miR-223. A. Representative western blots show that overexpression of the miR-223 mimic lowered IGF-1R protein levels compared to the controls and inhibitor. GAPDH was used as a loading control. B. Data were normalized to the expression of GAPDH and presented as expression relative to untreated cellular controls. C. The 3’-UTR of IGF-1R was fused to a Luciferase reporter and activity was assessed after treatment with the miR-223 mimic, miR-223 inhibitor, or negative controls. Luciferase activity was lower in cells overexpressing miR-223 compared to controls. Data are means ± SD from three experiments (**P < 0.01; *P < 0.05).