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Genotyping of wild-type (RGC32+/+), RGC32+/-, and RGC32-/- mice by PCR using genomic DNA isolated from mouse tails. 285 bp product alone indicates wild type. Presence of both 750 and 285 bp fragments indicate heterozygous, whereas 750 bp alone indicates the homozygous knockout. The genotype of No. 1, 4 and 5 mouse was RGC32+/+; the genotype of No. 2, 7, 8, 9, 12 mouse was RGC32+/-; the genotype of No. 3, 6, 10, 11 mouse was RGC32-/-.
