Figure 2.

LIPUS affects expression of proteins associated with proliferation and apoptosis in preadipocytes. The expression levels of key proliferation- and apoptosis-related molecules (PCNA, PPARγ, Bcl2, Bax, and C-C3) were examined by western blotting in primary preadipocytes with or without LIPUS (1700 cycles, 1 min) treatment. A, B. The protein levels of PCNA protein decreased and the levels of PPARγ increased in LIPUS treated cells compared to untreated cells. C, D. LIPUS treatment downregulated the protein expression of Bcl2 and the Bcl2/Bax ratio, and upregulated the protein expression of C-C3. All values are expressed as the mean ± SEM of three independent trials. Data were analyzed with independent t test. *P<0.05, **P<0.01.