Figure 4.

LIPUS induces phosphorylation of p38-MAPK. The expression and modification of MAPK members were assessed by western blotting in primary preadipocytes with or without LIPUS treatment. A. LIPUS treatment significantly promoted the phosphorylation level of p38 and inhibited the phosphorylation of ERK, and no change of the expression level of p-JNK were observed. B. Quantification of p-38, p-p38, ERK, p-ERK, JNK, and p-JNK protein levels were. Data were analyzed with independent t test. All values are expressed as the mean ± SEM of three independent trials. *P<0.05.