Figure 5.

Inhibition of p38 phosphorylation rescues the effects of LIPUS. In order to explore whether the biological effects of LIPUS on preadipocytes are associated with p-p38, cell suspension exposed to LIPUS was treated with or without p38 MAPK inhibitor SB203580. A, B. p38 inhibition rescued the LIPUS-induced apoptosis as shown by flow cytometric analysis. The percentage of early apoptotic preadipocytes (%) was analyzed with one-way ANOVA analysis. C. CCK-8 assay showed that inhibition of p38 activation reversed the LIPUS-induced proliferation inhibition in primary preadipocytes. D. p-p38, p-ERK, C-C3, PCNA and Bcl2 protein levels were assessed by western blotting with or without p38 inhibitor treatment. Relative protein levels were analyzed with one-way ANOVA analysis. All values are expressed as the mean ± SEM of three independent trials. *P<0.05.