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. 2018 Mar 15;10(3):989–997.

Figure 2.

Figure 2

MiRNA-134-5p is upregulated in HG-treated podocytes and promotes podocyte apoptosis. A. The expression of miR-134-5p in podocytes treated with HG (30 mM) at different time points (0-72 h) was analyzed by qRT-PCR. B. The expression of miR-134-5p was analyzed by qRT-PCR after anti-miR-134-5p transfection in HG-treated podocytes. C. The relative mRNA level of nephrin was analyzed by qRT-PCR in HG-treated podocytes after transfection with anti-miR-ctrl or anti-miR-134-5p. D, E. The expression level of nephrin in HG-treated podocytes was analyzed by western blotting. F, G. Apoptosis in HG-treated podocytes was measured by western blot analysis and flow cytometry analysis of Annexin V and PI double staining. **P < 0.01. Data are presented as the mean ± SE.