Table 1.
Comparison of input volumes of HPV genotyping assays.
| HPV genotyping assay | Nucleic acid extraction method | Original sample input volume (mL) | Elution volume (mL) | PCR template volume (mL) | Total post-PCR volume (mL) | Total detection volume (mL) | Fraction of original sample detected | Equivalent original sample volume tested (mL) |
|---|---|---|---|---|---|---|---|---|
| This study: | ||||||||
| Anyplex IIa | MagNAPure 96 | 0.50a | 0.05 | 0.005 | 0.01 | 0.01 | 0.1 | 0.05 |
| Linear Arraya | MagNAPure 96 | 1.00a | 0.10 | 0.05 | 0.20 | 0.075 | 0.05 | 0.047b |
| EuroArraya | MagNAPure 96 | 0.50 | 0.05a | 0.005 | 0.09 | 0.065 | 0.01 | 0.007 |
| Manufacturers’ recommendations: | ||||||||
| Anyplex II | various | 1.00 | 0.05 – 0.10 | 0.005 | 0.01 | 0.01 | 0.05 – 0.10 | 0.05 – 0.10 |
| Linear Array | AmpliLute Liquid Media Extraction | 0.25 | 0.10 | 0.05 | 0.20 | 0.075 | 0.05 | 0.012b |
| EuroArray | QIAamp or NucleoSpin | 0.50 | 0.06 – 0.10 | 0.005 | 0.09 | 0.065 | 0.007 – 0.012 | 0.004 – 0.006 |
a
Volumes apply to this study and are a deviation from the manufacturer's recommendations.
b
Extraction volumes have been validated to ensure equivalent sensitivity using DNA extracted by MagNAPure 32 automated system compared with the manufacturer-specified AmpliLute manual system.