Table 1.
Crude extracts preparation yields and activity parameters
| Plant | Part | Solvent | Extract code | Extraction yield (%) | IC50 ± SD (µg/mL) | RI | CC50 (µg/mL) | Selectivity Index | ||
|---|---|---|---|---|---|---|---|---|---|---|
| PfINDO | Pf3D7 | PfINDO | Pf3D7 | |||||||
| T. mantaly | Leaf | Water | Tmlw | 35.50 | 2.09 ± 0.06 | 2.66 ± 0.31 | 0.79 | > 200 | > 95.69 | > 75.19 |
| Methanol | Tmlm | 19.54 | 2.69 ± 0.04 | 2.61 ± 0.43 | 1.03 | > 200 | > 74.35 | > 76.63 | ||
| Stem bark | Water | Tmsbw | 19.93 | 0.26 ± 0.02 | 1.03 ± 0.04 | 0.25 | > 200 | > 769.23 | > 194.17 | |
| Methanol | Tmsbm | 19.32 | 3.63 ± 0.50 | 2.91 ± 0.12 | 1.25 | > 200 | > 55.10 | > 68.73 | ||
| Root | Water | Tmrw | 07.45 | > 25 | > 25 | – | – | – | – | |
| Methanol | Tmrm | 09.01 | 7.01 ± 0.82 | 5.04 ± 0.68 | 1.39 | > 200 | > 28.53 | > 39.68 | ||
| T. superba | Leaf | Water | Tslw | 17.18 | 0.57 ± 0.06 | 1.26 ± 0.12 | 0.45 | > 200 | > 350.88 | > 158.73 |
| Methanol | Tslm | 12.07 | 3.38 ± 0.54 | 2.13 ± 0.13 | 1.59 | > 200 | > 59.17 | > 93.90 | ||
| Stem bark | Water | Tssbw | 14.15 | 3.70 ± 0.03 | 1.42 ± 0.09 | 2.61 | > 200 | > 54.05 | > 140.85 | |
| Methanol | Tssbm | 10.35 | 2.68 ± 0.20 | 1.85 ± 0.13 | 1.44 | > 200 | > 74.63 | > 108.11 | ||
| Root | Water | Tsrw | 04.54 | 18.88 ± 0.14 | 16.43 ± 0.14 | 1.15 | – | – | – | |
| Methanol | Tsrm | 02.27 | 2.38 ± 0.08 | 2.28 ± 0.20 | 1.04 | > 200 | > 84.03 | > 87.72 | ||
| Reference drug | Chloroquine (µM) | – | 0.40 ± 0.00 | 0.04 ± 0.01 | 10.00 | – | – | – | ||
Activity data are presented as means of triplicate experiments
IC50: 50% inhibitory concentration; CC50: 50% cell cytotoxic concentration, cytotoxicity was tested against HEK239T cells; SD: standard deviation from triplicate experiments; RI: resistance index; resistance index was calculated as the ratio of IC50 PfINDOresistant/IC50Pf3D7sensitive; SI: selectivity index; Tm: Terminalia mantaly; Ts: Terminalia superba; l: leaf; sb: stem bark; r: root; w: water; m: methanol