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. 2018 Apr 3;19:17. doi: 10.1186/s12868-018-0420-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Human dopaminergic neurons express Dopamine Transporter. A. Representative western blot (A) and optical density (B) of dopamine transporter (DAT) show the presence of DAT in the lysates from vehicle and 27-OHC-treated neurons. Immunofluorescence imaging shows immunopositive staining for DAT in untreated neurons (C; green). Immunofluorescence for the neuron specific β-III Tubulin marker (D; red) and for nuclear counterstaining with DAPI (E; blue). F Overlay of dopamine transporter, neuron specific β-III Tubulin, and DAPI showing both nuclear and cytoplasmic localization of DAT (arrows)