Fig. 6.

The clinical significance HMGB1 in pancreatic cancer patients. a IHC staining on serial sections of pancreatic carcinoma patient gross specimen showed an overlap expression of Caspase-3, MMP9, and HMGB1 in the necrosis area. The necrosis area was detected by HE staining. Caspase-3, MMP9, and HMGB1 location and expression level were accessed by IHC. Magnification: × 4 and × 10. b Analysis of the TCGA database indicates that the expression level of HMGB1, Caspase-3, and EMT-related proteins were highly consistent. The results are presented by heat map: n = 168. c Analysis of the TCGA database indicates that HMGB1 expression is correlated with patient survival: n = 84 for HMGB1-low group; and n =84 for HMGB1-high group. d The HMGB1 level in serum was higher following radiotherapy and highly related with TNM staging. *p < 0.05, **p < 0.01, ***p < 0.001