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. 2018 Mar 13;114(5):1128–1141. doi: 10.1016/j.bpj.2018.01.009

Figure 2.

Figure 2

SH-SY5Y cell surface area. (A) Optical sections of a fixed cell visualized by TMA-DPH are shown. For clarity, only every fifth image of a stack of 28 images is shown. Scale bar, 10 μm. (B) 3D cell morphology reconstructed from the entire image stack. Scale bar, 5 μm. (C) From cells reconstructed as illustrated in (B), we determined the average imaged cell surface area to be 1256 ± 254 μm2 (value is given as mean ± SD; n = 3 independent experimental days with at least 19 cells per experiment). The black arrow in (C) marks the cell shown in (B). To see this figure in color, go online.