Figure 3.

Overlap between APP antibody staining and overexpressed APP-GFP. (A) On the left, GFP-signal was detected on plasma membranes generated from APP₆₉₅-GFP overexpressing cells; on the right, the corresponding immunostaining fluorescence obtained by APP antibody staining is shown. Scale bar, 4 μm. (B) A zoomed-in view of an overlay from the bottom parts of the images shown in (A) is shown, with a line scan (dashed white line) illustrating the high similarity between the GFP- and antibody-staining signals. Overlap was quantified by calculation of the Pearson correlation coefficient (PCC). The PCC value is displayed in the image as mean ± SD (n = 3 independent experiments with at least 22 sheets per experiment). To see this figure in color, go online.