Figure 3.
Kratky plots of scattering profiles. (A) Typical Kratky plots of IDPs are shown with different degrees of compactness. (B) A Kratky plot is shown with signs for both compaction (red shading) and expansion (blue shading). The mixing of compaction and expansion can occur in the same molecule (lower-left cartoon) or in two populations of molecules (lower-right cartoon). (C) Kratky plots are presented for FlgM in buffer and under protein crowding or polymer crowding. I(0) and Rg values from GNOM were used for making the abscissa and ordinate dimensionless. Crowder concentrations in mg/mL are indicated in the legends. To see this figure in color, go online.