Figure 6.

Transformation of a coarse-grained cubic-phase glycosylceramide structure into an all-atom structure enables comparison to original cryo-EM data. The glycosylceramide structure with a hydration of ∼14.5 waters/lipid was transformed into an all-atom structure (top). To compare to experimental cryo-EM data (a), the unit cell of the all-atom structure was extended in three dimensions (b) and subjected to a cryo-EM simulation. Features from selected parts of experimental cryo-EM images (d) can then be compared to features present in the simulated cryo-EM images (e), or toward a negative control such as water (c and f). (a and d) adapted from Norlén, unpublished data, with permission. To see this figure in color, go online.