Figure 7.

Transformation of a lamellar coarse-grained ceramide structrure into an all-atom structure enables comparison to the original cryo-EM data. The lamellar ceramide structure with a hydration of ∼14.5 waters per lipid (Fig. 4f) was transformed into an all-atom structure (top). To compare to experimental cryo-EM data (a), the unit cell of the all-atom structure was extended in three dimensions (b) and subjected to a cryo-EM simulation. Features from selected parts of the experimental cryo-EM images (c) can then be compared to features present in the simulated cryo-EM images (d). Images (a) and (c) are adapted from Norlén, unpublished data, with permission. To see this figure in color, go online.