Figure 3.

Effect of MAP2c on the pressure stability of active microtubules. (A) Pressure-dependent and azimuthally averaged Synchrotron SAXS data for active microtubules at 37°C and bound to MAP2c. The microtubules are polymerized in the presence of MAP2c with a 1:5 molar ratio of MAP2c to αβ-tubulin. The dash-dotted line shows the theoretical scattering profile of αβ-tubulin (PDB: 1JFF). (B) Comparison between experimental scattering profiles of active microtubules in the absence and presence of MAP2c and theoretical form factors of microtubules consisting of different numbers of protofilaments or featuring increased wall thickness. For better comparison, the scattering profiles are normalized to equal height of the first peak maxima. To see this figure in color, go online.