Skip to main content
. 2018 Feb 22;20(3):295–303. doi: 10.1016/j.neo.2018.01.005

Figure 2.

Figure 2

EMT occurred during apoptosis reversal both in vitro and in vivo.

(A) H&E-stained sectioned organs from tumor-bearing mice in the 50,000-injected-cell groups versus normal mice. Scale bar: 100 μm. (B) Immunofluorescence images of E-cadherin (green) and N-cadherin (red) in reversed (top), solvent control (middle) and untreated (bottom) MCF-7 xenografts. Scale bar: 25 μm. (C) H&E-stained and immunofluorescence images showing a tumor (T) in the lymph node (LN) from the reversed MCF-7 group (top) and a normal lymph node (bottom). Dash line indicates the LN-T boundary. Scale bar: 50 μm (H&E); 25 μm (confocal). (D) Immunofluorescence images showing the switch from epithelial (E-cadherin, green) to mesenchymal marker (N-cadherin, magenta) in reversed cells. Scale bar: 25 μm. (B-D) Nuclei were stained with DAPI. (E) Relative expressions of mesenchymal markers in the reversed and solvent control cells. Data represent mean±SEM (n = 3). **P < .01; ***P < .001.