Figure 4.

Effect of GFP-Siah1a-CT on mGluR-mediated calcium current modulation. A and C, Sample current traces illustrating calcium current before and during inhibition by 100 μM glutamate in cells expressing mGluR5b (A) and mGluR5b + Siah1a (C). Voltage protocol was as in figure 1. The scale bars indicate 20 msec and 0.5 nA (A) or 1 nA (C). Tail currents were cropped to better illustrate the step currents. B and D, Time course of inhibition by glutamate (Glu) in the cells illustrated in A and C, respectively. Closed circles represent measurements taken from the prepulse (the first test pulse to +10 mV). Open circles represent measurements taken from the postpulse (the second test pulse to +10mV).