Fig. 3.

Protection effects of BME on fatty liver induced by HF/HFrD. (a) HF (containing 60% fat)/HFr (containing 30% fructose) water was administered to C57BL/6J mice for 7 weeks. Subsequently, the mice were randomly divided into two groups (n = 6 mice/group). Each group was orally administered either DMSO or BME (100 mg/kg) on every other day for 5 weeks under the HF/HFr diet. (a) After 5 weeks, GTT was then carried out by measuring glucose levels at 0, 0.25, 0.5, 1, and 2 h after glucose injection (1 g/kg). Livers were isolated from HF/HFr and HF-/HFr-/BME-fed mice. Data are presented as mean ± SEM (n = 6 per group). *p < 0.05, **p < 0.01 versus HF/HFr group. (b) Liver sections stained with hematoxylin & eosin and Oil Red O. (c) Liver TG was extracted using the Folch extraction method and TG level was then measured with a TG assay kit. (d) Levels of plasma ALT, TG, cholesterol, and FFA were measured using an autochemical analyzer. Data are presented as mean ± SEM (n = 6 per group). **p < 0.01 versus HF/HFr group (c, d).