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miR-431 prevents synapse loss in cortico-hippocampal cultures of 3-month 3xTg mice. Cultures of 3-month 3xTg mice transfected with miR-431 demonstrated significant increase in (A) pre-synaptic puncta and (B) post-synaptic puncta in comparison tp cultures treated with Dkk1, relative to control. (C) Average neurite length. *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001.
