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Prevention of synapse loss in cortico-hippocampal cultures of 6-month 3xTg mice. Cortico-hippocampal cultures of 6-month 3xTg mice transfected with miR-431 demonstrated significantly higher number of (A) pre-synaptic puncta and (B) post-synaptic puncta in comparison to cultures treated with Dkk1. (C) Number of branches; (D) neurite length. *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001.
