Figure 5.

qRT-PCR analysis of Wnt signaling proteins after AβDDL and miR-431 treatments of cortico-hippocampal cultures of WT mice. Cortico-hippocampal cultures were transfected with miR-431 or a negative miRNA mimic control 48 h prior to treatment with AβDDL. 24 h after treatment neurons were collected and extracted RNA was used for qRT-PCR. Control cultures were untreated. As an internal control for PCR, primers for S12 were added for RNA template normalization. The relative quantifications of gene and miRNA expression were calculated against S12 by the ΔΔCt 2 method.