Figure 6.

Representative immunofluorescent images of the performed experiments. (A–C) Illustrate effects of Aβ treatment and miR-431 transfection on synaptic puncta visualized with anti-PSD95 antibodies (Texas Red). DAPI (blue) was used to visualize nuclei and FITC (green) to visualize stating with Tuj antibodies against neuron-specific tubulin. The synaptic puncta is reduced after Aβ treatment (B) and preserved following miR-431 transection (C). (D–F) Illustrate effects of Aβ treatment and miR-431 transfection on neurite length in cortico-hippocampal cultures visualized with Synapsin1 antibodies (FITC). The neurites degenerate after Aβ treatment (E) and protected following miR-431 transection (F). (G–J) Shows triple-staining of WT cortico-hippocampal culture with TUJ antibodies (G, green), anti-Krm1 antibodies (H, red) and nuclear stain DAPI (I, blue). (J) Shows overlay of (G–I). (K–M) Illustrate effect of miR-431 treatment of expression of Krm1. (K) Shows staining of untreated cortico-hippocampal culture with anti-Kremen antibodies. (L) Shows decreased expression of Krm1 after the culture was transfected with miR-431 for 48 h. (M) Graph shows relative reduction (percent change) in Krm1 fluorescent intensity after miR-431 treatment in comparison to untreated culture.