Table 3.
Expression of the oviduct, epithelial, and progenitor markers in oviduct tissue and cultured oviduct epithelial cells in hen and quail
| Gene panel | Gene | Hen | Quail | ||
|---|---|---|---|---|---|
| Tissuea | Cell cultureb | Tissuea | Cell cultureb | ||
| Oviduct markers | ESR1 | + | + | + | + |
| OVAL | + | + | + | ND | |
| OVM | + | + | + | ND | |
| Epithelial markers | KRT5 | + | + | + | + |
| KRT14 | + | + | + | + | |
| OCLN | + | + | + | + | |
| Stem-like/Progenitor markers | CD44 | + | + | + | + |
| LGR5 | ND | + | + | + | |
| MSI1 | + | + | + | + | |
| SOX9 | + | + | + | + | |
| NANOG | + | + | + | + | |
| OCT4/cPOUV | ND | + | + | + | |
aHen/quail oviduct tissue, divided into three fragments: INF infundibulum, DM distal magnum, and PM proximal magnum; bHen/quail oviduct epithelial cell culture derived from different parts of the oviduct (INF, DM, or PM) and cultured in vitro; “+” denotes positive result of RT-qPCR analysis (Ct < 35), meaning that the gene was expressed in a given sample. ND not detected