Figure 2.

Short-term drug response dynamics reveal preexisting clonal heterogeneity. (A) Population growth curves (log₂ normalized) for BRAFi-treated SKMEL5 single-cell-derived colonies (n = 203) obtained using the cFP assay are shown. (B) Shown is the distribution of clonal drug-induced proliferation (DIP) rates (doublings per hour) obtained by linear fits to the growth curves. (C) Colony DIP rates do not correlate with untreated proliferation rates. (D) The clonal composite (sum of cell counts from all colonies at each time point) closely corresponds to the short-term population-level response (same data as in Fig. 1A; means are shown as solid or dashed lines, and 95% confidence intervals are shown as shaded regions). Prolif., proliferation; UnTx, untreated. To see this figure in color, go online.