Figure 3.

Single-cell-derived subclone populations idle independent of short-term drug sensitivity. (A) Shown are population growth curves (log₂ normalized) for three single-cell-derived SKMEL5 subclones treated with a BRAFi (3+ technical replicates; means are shown as solid lines, and 95% confidence intervals are shown as shaded regions). (B) Shown are single-cell life spans versus birth times (time of first mitotic event) for the three subclones after a week in a BRAFi (cells born during the experiment but that reached the end of the experiment (EOE) without a second mitotic event are plotted along the diagonal). (C) Shown are population growth curves (log₂ normalized) for drug-naïve and post-idling (1 week of BRAFi treatment; the BRAFi was removed overnight (16–24 h) before subsequent drug addition) single-cell-derived SKMEL5 subclones treated with saturating concentrations of trametinib, cisplatin, BKM120, and BEZ235 (error bars represent one standard deviation). To see this figure in color, go online.