Figure 3.

Negative Gaussian curvature guides cell polarization and migration. (A) A projection of Lifeact-GFP signals of two cells on an SWS skirt is shown. Red concentric circles indicate rings of common height separated by 18 μm in height. The yellow dashed ring indicates the approximate location of the line of inflection. Magenta lines indicate trajectories of centers of mass. Cell outline colors indicate boundary velocities. Scale bar, 50 μm. (B) The radial distance of cell centers from the SWS symmetry axis is shown. The dashed line indicates the position of the line of inflection. (C) The radial distance of the boundary position closest to the SWS symmetry axis is shown. CBP, closest boundary point. The dashed line indicates the position of the line of inflection. n = 16 cells from three experiments. (D) The boundary velocity averaged over all time points for the bottom cell in (A) is shown. The red circle indicates zero velocity. (E) The average boundary velocities in different directions are shown. Boundaries toward cap include angles from 315 to 45°, leading edge from 45 to 135°, away from cap from 135 to 225°, and trailing edge from 225 to 315°. n = 13 cells from three experiments. The results are the mean and SE, ^∗∗∗p ≤ 0.001 (Student’s t-test). deg, degree. To see this figure in color, go online.