Figure 4.

Negative Gaussian curvature alters migration direction relative to SF orientation. (A) A projected Lifeact-GFP signal of a representative cell on a planar region of an SWS substrate is shown. At t = 0 h, the cell’s SFs are oriented in the horizontal direction (blue), and it migrates to the left (red). At t = 12 h, the same cell’s SFs are oriented in the vertical direction, and it migrates downward. (B) The SF and velocity angles for the cell shown in (A) are displayed. (C) A cell on an SWS skirt has SFs oriented in the horizontal direction (blue), but it migrates down and to the right (red). Red concentric circles indicate rings of common height separated by 18 μm in height. The yellow dashed circle indicates the approximate location of the line of inflection. (D) The SF and velocity angles for the cell shown in (C) are displayed. (E) The angle between SF and velocity vectors for cells on flat regions versus skirts is shown. n_flat = 19 cells from two experiments; n_skirt = 14 cells from three experiments. Scale bars, 50 μm. The results are the mean and SE, ^∗∗∗p ≤ 0.001 (Student’s t-test). deg, degree; hr, hour; Vel., velocity. To see this figure in color, go online.