Fig. 81.2.

Staining of ROS generation on ARPE-19 monolayers expressing endogenous and exogenous DJ-1 and exposed to oxidative stress by exposure to 800 µM H₂O₂ for 1 h. Incubation of RPE monolayers with carboxy-DCFDA indicates that no ROS is generated by the RPE cells at basal conditions (a). While upon oxidative stress incubation, appreciable ROS generation throughout the entire cell body of cells is observed (b). RPE cultures transduced with an adenovirus carrying the full-length hDJ-1 DNA did not display ROS generation when exposed to oxidative stress (c). However, RPE cultures transduced with an adenovirus carrying DJ-1 with the three cysteine at residues 46, 53, and 106 mutated to serine (d). Displayed appreciable ROS generation when cells were exposed to oxidative stress. Bar = 20 µm