Skip to main content
. Author manuscript; available in PMC: 2018 Jul 3.
Published in final edited form as: Nature. 2018 Jan 3;553(7687):212–216. doi: 10.1038/nature25168

Fig. 2. Functional heterogeneity of MPP lineage fates in steady state hematopoiesis.

Fig. 2

a, Chart shows the alignment of all active MPP tags together with the five analysed blood lineages at each time point (all tags collected from 3 mice per time point). LT-HSC tags were analysed in parallel and excluded from the analysis to represent only MPP behaviour. b, Fraction of active MPP tags that overlap with a single lineage (calculated independently for each lineage). Values are mean +/− s.e.m. from 3 mice. *pMk-Er=0.13, pMk-Gr=0.03, pMk-Mo=0.03, pMk-B=0.001 (8 wk). c, Distribution of Lin+ clone sizes comparing tags overlapping with MPP vs. non-overlapping at 8 wk. Values are median and interquartile ranges of all detected clones from 3 mice. *Kolmogorov-Smirnov pMk=0.03, pB=0.25, pEr=0.03, pGr=0.001, pMo=0.003. d, Fraction of each lineage replaced by MPPs calculated as the percentage of total MPP-overlapping lineage reads over time. Values are mean +/− s.e.m. from 3 independent mice. *pEr-Gr/Mo/B=0.04, pEr-Mk=0.03 (2 weeks) and pB-Er/Mk=0.03, pB-My=0.04 (8 weeks). e, Average number of detected active MPP clones per lineage per mouse at different time points (normalized for %DsRed labelling efficiency).