Figure 4.

LncRNA ZFAS1 directly interacted with miR-507 in CRC. (A) The predicted complementary sequence between miR-484 and ZFAS1 3’-UTR. (B) HEK293T cells were transfected with ZFAS1 3′-UTR Wt or Mut reporter plasmids along with miR-484 mimics, and then luciferase activity assays were performed. (C) Relative expression of miR-484 was measured by qRT-PCR in CRC tissues. (D) Relative expression of miR-484 was measured by qRT-PCR in CRC cells. (E) Pearson's correlation analysis between miR-484 and ZFAS1 expression in CRC tissues. (F) miR-484 expression in ZFAS1 knockdown CRC cells was determined by qRT-PCR. (G) Association of ZFAS1 and miR-484 with Ago2 in CRC cells. *p<0.05