Figure 4.

The effects of inhibitor LY294002 and inhibitor CI-1040 on the phosphorylation of AKT and MAPK and on VEGFC-induced proliferation of GC-1 cells. GC-1 cells were pretreated with or without LY294002 and CI-1040 for 30 min, and then VEGFC was added to the culture medium as described in the Materials and Methods. (A, B) Western blotting showed the phosphorylation of p44/p42 MAPK (MAPK) (A) and AKT (B) in GC-1 cells treated with or without VEGFC, LY294002 and CI-1040 compared with the negative control (upper panel), p44/p42 MAPK (MAPK) (A) and AKT(B) was used as a loading control for total proteins (lower panel). The right panels showed the ratio between phosphorylated AKT and MAPK with total AKT and MAPK protein. (C) CCK-8 assay revealed the quantification of the GC-1 cells treated with VEGFC, LY294002, or CI-1040 for 4 days. (*P<0.05, **P<0.01, ***P<0.001).