Figure 5.

VEGFC/VEGFR3 signaling stimulated the transcript of c-fos and Egr1 and increased the expression level of Cyclin D1. (A, B) Real-time PCR showed that the change of c-fos and Egr1 transcript after VEGFC treatment or VEGFR3 knockdown in GC-1 cells. (C) Western blotting showed the levels of cyclin A, cyclin D1 and cyclin E1 proteins in GC-1 cells transfected with VEGFR3 shRNA or negative shRNA followed by being treated with or without VEGFC. The right panels displayed the amount of proteins normalized with ACTB. Values in (A-C) were represented as mean ± SD from three independent experiments. (*P<0.05, **P<0.01).