Figure 7.

VEGFR3 was the primary receptor but not VEGFR2 for VEGFC to activate the phosphorylation of PI3K-AKT and MAPK. GC-1 cells were pretreated with anti-VEGFR2 or anti-VEGFR3 antibody for 30 min, and then VEGFC was added to the culture medium as described in the Materials and Methods. (A) Western blotting showed the phosphorylation of AKT and p44/P42 MAPK activated by VEGFC treatment after blocking VEGFR2 or VEGFR3 in GC-1 cells (upper panel). The expression levels of AKT and p44/P42 MAPK (MAPK) were used as a loading reference for total proteins (lower panel). (B) The ratios of phosphorylation for these proteins were showed in the form of histogram on the right. Values in (B) were represented as mean ± SD from three independent experiments. (*P<0.05, **P<0.01, ***P<0.001).