Figure 2.

Abundance and expression of AtVLN genes. A, Genomic DNA gel-blot hybridizations. Six micrograms of total Arabidopsis DNA digested with the indicated restriction enzymes were loaded per lane. The left blot was hybridized with a cDNA fragment of AtVLN 1 and the right blot with a cDNA fragment of AtVLN 3. B, RNA gel-blot hybridizations. One microgram of poly(A⁺) RNA was loaded per lane and the blot was hybridized with cDNA fragments as indicated. The blot was rehybridized with a genomic fragment of Arabidopsis ACTIN 1 as a loading control (AtACTI). The size of transcripts was approximately 3 kb for AtVLN and 1.6 kb for AtACT1.