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. 2018 Feb 20;11(2):426–435. doi: 10.1016/j.tranon.2018.01.022

Figure 5.

Figure 5

Superior in-vivo antiproliferative and apoptotic effects of nab-paclitaxel on esophageal adenocarcinoma tumor xenografts. OE19 cells were subcutaneously injected in athymic nude mice and after 2 weeks treated with carboplatin (CP), paclitaxel (PT) or nab-paclitaxel (NPT) as a monotherapy and in combinations for 2 weeks. Tumors harvested from five mice of OE19 xenografts for each treatments were processed and stained with cleaved caspase 3 (c-caspase 3) and Ki-67 antibodies. (A) Intratumoral apoptosis was measured by immunohistochemistry (IHC) of tissue sections for cleaved caspase 3. (B) Intratumoral proliferation was measured by IHC for Ki-67 nuclear antigen. (C) Cleaved caspase 3 (apoptosis index) and (D) Ki-67 (proliferative index) +ve cells were counted in five different high power fields for each treatments. Data are expressed as the percent mean ± standard deviation. * indicates p < 0.05 in PT versus NPT, ** indicates p < 0.05 in PT+CP versus NPT+CP (N = 5). Representative photomicrographs of cleaved caspase 3 (A) or Ki-67 (B) stained tumor sections (X40) were shown.