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. 2018 Mar 21;2018:2968252. doi: 10.1155/2018/2968252

Figure 3.

Figure 3

Knockdown of DAB2IP promotes gastric cancer cell growth and metastasis in vitro. (a, c) SGC7901 cells stably expressing the shRNA vector were maintained in culture media for 10 days and then fixed and stained with 0.1% crystal violet, and the colonies containing above 100 cells were counted manually. The representative photographs are presented (a; magnification, ×1), and the relative number of colonies was counted (c). The bands were quantified and presented as the mean ± SEM of three independent experiments. (b) Figures are curves of SGC7901 cell growth by MTT assays, presented as mean ± SEM (n = 5). (d) Migration assays were performed in wild-type cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP). Representative photographs are presented (left; magnification, ×200) and the relative number of migratory cells (right) was counted. The bands were quantified and presented as the mean ± SEM of three independent experiments. Statistical significance was determined by a two-tailed, unpaired Student t-test or one-way ANOVA. ∗∗P < 0.01; ∗∗∗P < 0.001.