Skip to main content
. 2018 Mar 19;2018:1023490. doi: 10.1155/2018/1023490

Figure 1.

Figure 1

ADCC mediated by PBMCs and NK92-derived cell lines. Percentages of maximal LDH release from target cells were determined with either PBMCs at 15-fold excess (a) or of two strains of genetically modified NK92 cells expressing CD16-176V at 5-fold excess (b, c) with or without 10 μg/ml of the indicated antibodies. (a) In six independent experiments 1.5∗105 JVM-3 or Mec1 target cells per well were incubated with PBMCs from a healthy donor with or without alemtuzumab for 4 hours. (b, c) In four independent assays each, LDH release from 3∗104 Raji cells was determined after 2 hours of coculture with the effector cell lines 26.5 or 1708-LC3E11 in the absence or presence of three different antibodies. LDH release from mixtures of effector and target cells without or with antibodies was compared by paired Student's t-test. In addition, the efficacy of the ADCC elicited by rituximab versus obinutuzumab was compared by paired t-test.∗p < 0.05; ∗∗p < 0.01.