Fig. 4.

Label free proteomics soleus from adult and old mice, (A) Heatmap of significantly changed proteins with fold change >1.5 and –10logP > 20 equivalent to P < 0.01 (B) Volcano plot of quantified protein changes with age and significance, proteins highlighted in red are significantly increased with age and proteins highlighted in green are decreased with age. Proteins highlighted in blue were antioxidant proteins that did not significantly change in abundance and were selected for immunoblotting. (C) Pathway analysis of quantitative proteomic data using PathVisio reveals a downregulation of proteins involved in mitochondrial electron transport chain.